Construction of an Agrobacterium Mediated RNAI Genetic Transformation Vector Targeting the Replicase Gene of Indian cassava mosaic virus and Evaluation of Their Transformation Ability in Cassava Immature Leaf Lobes

Cassava mosaic virus is one of the major problems affecting cassava industry in India. Currently there are no effective strategy to completely protect from viruses. In order attain resistance RNAi vectors targeting replicase gene Indian constructed this study. Their efficiency transform immature leaf lobes were also studied here. Replicase Tamilnadu cloned and sequenced. Conserved domains identified sub CSIRO vector system transformation studies done leaves. Two different constructed, utilizing a conserved 440bp 5’ end ICMV Rep (AC1) which corresponds part AC4 gene, functions as viral suppressor protein. The partial was sense anti-sense orientations intermediate vector, pHANNIBAL. After cloning into pHANNIBAL, cassettes released binary pART27, contains kanamycin-resistance plant selectable marker. use hygromycin selection agent genetic transformation, RNAi–Rep pCAMBIA1305.2. These constructs named pICR1 pICR2 respectively. Genetic leaves using could generate PCR positive plants. An agrobacterium mediated developed that can be transformed lobes. silence should further.